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KMID : 0358319960370121339
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Korean Journal of Urology
1996 Volume.37 No. 12 p.1339 ~ p.1344
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Effect of Transduced Tumor Necrosis Factor-alpha Gene Experession on Growth of Human Bladder Tumor Cell Lines
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Abstract
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Objective:
@EN To evaluate the effect of transduced tumor necrosis factor-α(TNF-α) gene expression on growth of human bladder tumor cell lines in vitro.
@ES materials and Methods:
@EN The complete cDNA of TNF-α was introduced to three human bladder tumor cell lines(T24, J-82, HT-1197) using a retroviral vector, a recombinant form of Molony murine kleukemia virus with TNF-α and Neo gene and transfected cells were selected
by
exposure to neomycin analog G418. Gene transfer and expression were confirmed by polymerase chain reaction9PCR) and reverse transcription polymerase chain reaction(RT-PCR)-Southern blotting. Cell growth was measured by MTT assay.
@ES Results:
@EN Successful gene transfer and expression were confirmed in all three cell bladder tumor lines. Growth of transfectd cells were compared with parental cell lines and no differences were found in all three lines(p>0.05).
@ES Conclusion:
@EN Expression of transduced TNF-α gene could not show any effect on growth of human bladder tumor cells in vitro.
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